Journal: Cell reports

Article Title: TRIM67 regulates exocytic mode and neuronal morphogenesis via SNAP47

doi: 10.1016/j.celrep.2021.108743

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit monoclonal anti-Syntaxin-1 , Santa Cruz , Sc-12736; RRID:AB_2271330.

Techniques: Recombinant, Transfection, Mass Spectrometry, Plasmid Preparation, Software

Journal:

Article Title: Promiscuous interaction of SNAP-25 with all plasma membrane syntaxins in a neuroendocrine cell

doi: 10.1042/BJ20050583

Figure Lengend Snippet: (A) Cells were co-immunostained using mouse anti-SNAP-25 and rabbit anti-syntaxin-1–4 (Syx1–Syx4) (B) Co-immunostaining of PC12 cells using mouse anti-SNAP-25 and rabbit SNAP-23. (C) Cells were co-immunostained using HPC-1 monoclonal anti-syntaxin-1 antibody and either anti-SNAP-23 or anti-SNAP-25 antibodies. Scale bar, 10 μm.

Article Snippet: Mouse anti-syntaxin-1 (clone HPC-1) and rabbit anti-syntaxin-3 antibodies were from Sigma (Gillingham, Kent, U.K.).

Techniques: Immunostaining

Journal:

Article Title: Promiscuous interaction of SNAP-25 with all plasma membrane syntaxins in a neuroendocrine cell

doi: 10.1042/BJ20050583

Figure Lengend Snippet: Cells were co-immunostained using mouse anti-syntaxin-1 (Syx1) and rabbit anti-syntaxin-2–4 (Syx2–Syx4). The rectangular areas shown in the bottom panels in (A) are magnified in the respective panels in (B) [scale bar in (A), upper left panel, 10 μm; scale bars in (B), 1 μm].

Article Snippet: Mouse anti-syntaxin-1 (clone HPC-1) and rabbit anti-syntaxin-3 antibodies were from Sigma (Gillingham, Kent, U.K.).

Techniques:

Journal:

Article Title: Promiscuous interaction of SNAP-25 with all plasma membrane syntaxins in a neuroendocrine cell

doi: 10.1042/BJ20050583

Figure Lengend Snippet: (A) Western immunoblot analysis of rat internal organs and different parts of brain. Protein normalization (30 μg per lane) was verified by Coomassie staining (CS). (B) Recombinant syntaxins 3 and 4 form SDS-resistant SNARE complexes upon addition of SNAP-25 and synaptobrevin 2 (syb2) in a 30 min reaction at 24 °C. Coomassie stained gel. (C) Syntaxins 3 and 4 (Syx3 and Syx4) are as capable of direct binding to SNAP-25 as syntaxin 1. Immobilized GST fusion proteins of syntaxins were incubated with SNAP-25 for 30 min and, after extensive washing, were analysed by SDS/PAGE and Sypro Orange staining. A control reaction was performed using glutathione–Sepharose beads without GST–syntaxin, but in the presence of SNAP-25.

Article Snippet: Mouse anti-syntaxin-1 (clone HPC-1) and rabbit anti-syntaxin-3 antibodies were from Sigma (Gillingham, Kent, U.K.).

Techniques: Western Blot, Staining, Recombinant, Binding Assay, Incubation, SDS Page

Journal: Journal of Proteomics

Article Title: Novel pathophysiological markers are revealed by iTRAQ-based quantitative clinical proteomics approach in vascular dementia

doi: 10.1016/j.jprot.2014.01.011

Figure Lengend Snippet: Significantly regulated proteome of VaD selected from the complete list of confidently identified proteins a .

Article Snippet: WB was performed after SDS-PAGE by probing with primary antibodies at the indicated dilutions: anti-ACTB (beta-actin, 1:10 000, mouse monoclonal; Millipore, Billerica, MA, USA), anti-BCL2 (B-cell CLL/lymphoma 2, 1:1000, mouse monoclonal; Santa Cruz Biotech, Santa Cruz, CA, USA), anti-ferritin (1:4000, rabbit polyclonal; Abcam, Cambridge, UK), anti-HSPA4 (heat shock 70 kDa protein 4, 1:5000, mouse monoclonal; Abcam), anti-ICAM5 (intercellular adhesion molecule 5, 1:1000, rabbit polyclonal; Abcam), anti-NCAM (neural cell adhesion molecule, 1:10 000, rabbit polyclonal; Santa Cruz Biotech), anti-PEA15 (phosphoprotein enriched in astrocytes, 1:800, rabbit polyclonal; Abcam), anti-SOD1 (superoxide dismutase 1, 1:2000, rabbit polyclonal; Abcam), anti-syntaxin (1:10 000, mouse monoclonal; Milipore), anti-SYNPO (synaptopodin, 1:1000, rabbit polyclonal; Abcam) and anti-VDAC1 (voltage-dependent anion channel 1, 1:5000, mouse monoclonal; Santa Cruz Biotech).

Techniques: Transmission Assay